Chapter 02 Microscopy

Chapter 02 Microscopy

Complete Chapter Questions With Answers

Sample Questions Are Posted Below

Fill in the Blank Questions

1. The __________ is the point at which a lens focuses parallel beams of light.
   focal point

ASM Objective: 08.01 Properly prepare and view specimens for examination using microscopy (bright field and, if possible, phase contrast).
ASM Topic: Module 08 Microbiology Laboratory Skills
Blooms Level: 1. Remember
Learning Outcome: 02.01.02 Correlate lens strength and focal length
Section: 02.01
Topic: Microscopy

2. The __________ is the distance between the center of a lens and the point at which it focuses parallel beams of light.
   focal length

ASM Objective: 08.01 Properly prepare and view specimens for examination using microscopy (bright field and, if possible, phase contrast).
ASM Topic: Module 08 Microbiology Laboratory Skills
Blooms Level: 1. Remember
Learning Outcome: 02.01.02 Correlate lens strength and focal length
Section: 02.01
Topic: Microscopy

True / False Questions
 

3. Light rays are refracted (bent) when they cross the interface between materials with different refractive indices.
   TRUE

ASM Objective: 08.01 Properly prepare and view specimens for examination using microscopy (bright field and, if possible, phase contrast).
ASM Topic: Module 08 Microbiology Laboratory Skills
Blooms Level: 1. Remember
Learning Outcome: 02.01.01 Relate the refractive indices of glass and air to the path light takes when it passes through a prism or convex lens
Section: 02.01
Topic: Microscopy

Multiple Choice Questions

4. Confocal microscopes exhibit improved contrast and resolution by
   A.illumination of a large area of the specimen.
   B. blocking out stray light with an aperture located above the objective lens.
   C. use of light at longer wavelengths.
   D. use of ultraviolet light to illuminate the specimen.

ASM Objective: 08.01 Properly prepare and view specimens for examination using microscopy (bright field and, if possible, phase contrast).
Blooms Level: 2. Understand
Learning Outcome: 02.02.01 Evaluate the parts of a light microscope in terms of their contributions to image production and use of the microscope
Section: 02.02
Topic: Microscopy

5. A 30´ objective and a 20´ ocular produce a total magnification of
   A.230´.
   B. 320´.
   C. 50´.
   D. 600´.

ASM Objective: 08.01 Properly prepare and view specimens for examination using microscopy (bright field and, if possible, phase contrast).
ASM Topic: Module 08 Microbiology Laboratory Skills
Blooms Level: 3. Apply
Learning Outcome: 02.02.01 Evaluate the parts of a light microscope in terms of their contributions to image production and use of the microscope
Learning Outcome: 02.02.02 Predict the relative degree of resolution based on light wavelength and numerical aperture of the lens used to examine a specimen
Section: 02.02
Topic: Microscopy

6. A 45´ objective and a 10´ ocular produce a total magnification of
   A.900´.
   B. 55´.
   C. 450´.
   D. 145´.

ASM Objective: 08.01 Properly prepare and view specimens for examination using microscopy (bright field and, if possible, phase contrast).
ASM Topic: Module 08 Microbiology Laboratory Skills
Blooms Level: 3. Apply
Learning Outcome: 02.02.01 Evaluate the parts of a light microscope in terms of their contributions to image production and use of the microscope
Section: 02.02
Topic: Microscopy