Chapter 18 Microbial Genomics

Chapter 18 Microbial Genomics

Complete Chapter Questions With Answers

Sample Questions Are Posted Below

True / False Questions

1. Genomic studies of marine microorganisms has revealed that a considerable amount of the photosynthesis in the ocean is based on photopigments called proteorhodopsins.
   TRUE

ASM Objective: 05.03 Microorganisms and their environment interact with and modify each other.
ASM Objective: 06.02 Microorganisms provide essential models that give us fundamental knowledge about life processes.
ASM Objective: 07.01b Ability to apply the process of science: Analyze and interpret results from a variety of microbiological methods and apply these methods to analogous situations.
ASM Topic: Module 04 Information Flow and Genetics
Blooms Level: 3. Apply
Learning Outcome: 18.03.02 List two applications of metagenomics in any field of microbial biology
Section: 18.03
Topic: Genotypic Methods for Identification

Fill in the Blank Questions

2. The method of nucleotide sequence determination that utilizes dideoxynucleotides was developed by _________.
   Sanger

ASM Objective: 05.03 Microorganisms and their environment interact with and modify each other.
ASM Objective: 06.02 Microorganisms provide essential models that give us fundamental knowledge about life processes.
ASM Objective: 07.01b Ability to apply the process of science: Analyze and interpret results from a variety of microbiological methods and apply these methods to analogous situations.
ASM Topic: Module 04 Information Flow and Genetics
Blooms Level: 2. Understand
Learning Outcome: 18.01.01 Explain how DNA is sequenced by the Sanger chain termination method
Section: 18.01
Topic: Genetic Analyses

True / False Questions
 

3. Modern automated sequencing machines detect DNA bands by measuring incorporated radioactivity.
   FALSE

ASM Objective: 05.03 Microorganisms and their environment interact with and modify each other.
ASM Objective: 06.02 Microorganisms provide essential models that give us fundamental knowledge about life processes.
ASM Objective: 07.01b Ability to apply the process of science: Analyze and interpret results from a variety of microbiological methods and apply these methods to analogous situations.
ASM Topic: Module 04 Information Flow and Genetics
Blooms Level: 2. Understand
Learning Outcome: 18.01.01 Explain how DNA is sequenced by the Sanger chain termination method
Section: 18.01
Topic: Genetic Analyses

4. Dideoxynucleotide sequencing methodologies rely on the ability of dideoxynucleotides to facilitate chain extension after incorporation by DNA polymerase.
   FALSE

ASM Objective: 05.03 Microorganisms and their environment interact with and modify each other.
ASM Objective: 06.02 Microorganisms provide essential models that give us fundamental knowledge about life processes.
ASM Objective: 07.01b Ability to apply the process of science: Analyze and interpret results from a variety of microbiological methods and apply these methods to analogous situations.
ASM Topic: Module 04 Information Flow and Genetics
Blooms Level: 2. Understand
Learning Outcome: 18.01.01 Explain how DNA is sequenced by the Sanger chain termination method
Section: 18.01
Topic: Genetic Analyses

Multiple Choice Questions
 

5. Each of the four Sanger sequencing reactions include
   A.a combination of the four different dideoxynucleotides.
   B. a single dideoxynucleotide triphosphate.
   C. ATP.
   D.  tRNA.

ASM Objective: 05.03 Microorganisms and their environment interact with and modify each other.
ASM Objective: 06.02 Microorganisms provide essential models that give us fundamental knowledge about life processes.
ASM Objective: 07.01b Ability to apply the process of science: Analyze and interpret results from a variety of microbiological methods and apply these methods to analogous situations.
ASM Topic: Module 04 Information Flow and Genetics
Blooms Level: 2. Understand
Learning Outcome: 18.01.01 Explain how DNA is sequenced by the Sanger chain termination method
Section: 18.01
Topic: Genetic Analyses