Chapter 29 Methods in Microbial Ecology

Chapter 29 Methods in Microbial Ecology

Complete Chapter Questions With Answers

Sample Questions Are Posted Below

Multiple Choice Questions

1. What percentage of microbes have been cultured in the laboratory?
   A.1%
   B. 10%
   C. 20%
   D. 30%

ASM Objective: 06.02 Microorganisms provide essential models that give us fundamental knowledge about life processes.
ASM Objective: 06.04 Because the true diversity of microbial life is largely unknown, its effects and potential benefits have not been fully explored.
ASM Objective: 08.03 Use appropriate methods to identify microorganisms (media-based, molecular and serological).
ASM Topic: Module 08 Microbiology Laboratory Skills
Blooms Level: 2. Understand
Learning Outcome: 29.01.01 Explain why, to the best of our knowledge, most microorganisms resist culturing in the laboratory
Section: 29.01
Topic: Microbial Ecology

True / False Questions

2. In the microdroplet culture method, cells from mixed assemblages are encapsulated in a gel matrix. The matrix is then emulsified to generate gel “microdroplets”; each microdroplet contains a mixture of cells.
   FALSE

ASM Objective: 06.02 Microorganisms provide essential models that give us fundamental knowledge about life processes.
ASM Objective: 06.04 Because the true diversity of microbial life is largely unknown, its effects and potential benefits have not been fully explored.
ASM Objective: 08.03 Use appropriate methods to identify microorganisms (media-based, molecular and serological).
ASM Topic: Module 08 Microbiology Laboratory Skills
Blooms Level: 2. Understand
Learning Outcome: 29.01.04 Describe at least two innovative approaches used to coax growth in the laboratory of previously uncultured microbes
Section: 29.01
Topic: Microbial Ecology

Fill in the Blank Questions

3. A technique used to quantify specific types of microbes in natural samples, enrichment cultures, and food or water samples is known as the _______ ________ _______ technique.
   most probable number

ASM Objective: 06.02 Microorganisms provide essential models that give us fundamental knowledge about life processes.
ASM Objective: 06.04 Because the true diversity of microbial life is largely unknown, its effects and potential benefits have not been fully explored.
ASM Objective: 08.05 Use appropriate microbiological and molecular lab equipment and methods.
ASM Topic: Module 08 Microbiology Laboratory Skills
Blooms Level: 2. Understand
Learning Outcome: 29.01.03 State when and how to apply the most probable number (MPN) method
Section: 29.01
Topic: Microbial Ecology

4. _______ culture are pure cultures, consisting of a single species.
   Axenic

ASM Objective: 06.02 Microorganisms provide essential models that give us fundamental knowledge about life processes.
ASM Objective: 06.04 Because the true diversity of microbial life is largely unknown, its effects and potential benefits have not been fully explored.
ASM Objective: 08.03 Use appropriate methods to identify microorganisms (media-based, molecular and serological).
ASM Topic: Module 08 Microbiology Laboratory Skills
Blooms Level: 1. Remember
Learning Outcome: 29.01.01 Explain why, to the best of our knowledge, most microorganisms resist culturing in the laboratory
Section: 29.01
Topic: Microbial Ecology

True / False Questions

5. The use of modern molecular techniques has provided a mechanism for growing the microorganisms previously referred to as unculturable.
   FALSE

ASM Objective: 06.02 Microorganisms provide essential models that give us fundamental knowledge about life processes.
ASM Objective: 06.04 Because the true diversity of microbial life is largely unknown, its effects and potential benefits have not been fully explored.
ASM Objective: 08.03 Use appropriate methods to identify microorganisms (media-based, molecular and serological).
ASM Topic: Module 08 Microbiology Laboratory Skills
Blooms Level: 2. Understand
Learning Outcome: 29.01.04 Describe at least two innovative approaches used to coax growth in the laboratory of previously uncultured microbes
Section: 29.01
Topic: Microbial Ecology