Chapter 3 Recombinant DNA Technology and Genomics

Chapter 3   Recombinant DNA Technology and Genomics

Complete Chapter Questions With Answers

Sample Questions Are Posted Below

1) You just cloned a new gene from mice. Which of the following techniques would be the best choice to use if you wanted to determine the number of copies of this gene in the mouse genome?

1. A) Northern blot analysis
2. B) RT-PCR
3. C) Western blot analysis
4. D) Southern blot analysis
5. E) In situ hybridization

Answer:  D

2) Which of the following techniques involves hybridizing a cDNA sample to a chip containing thousands of single-stranded DNA sequences, allowing one to study the expression of thousands of genes simultaneously?

1. A) PCR
2. B) Southern blot
3. C) FISH
4. D) Agarose gel electrophoresis
5. E) DNA microarray

Answer:  E

3) When making a complementary DNA (cDNA) library, which enzyme is used to copy mRNA into DNA?

1. A) DNA ligase
2. B) DNA polymerase
3. C) Primase
4. D) RNA polymerase
5. E) Reverse transcriptase

Answer:  E

4) In a recombinant DNA experiment, which enzyme is used to join together DNA fragments by forming phosphodiester bonds between nucleotides? Recall that this same enzyme joins together Okazaki fragments on the lagging strand during DNA replication.

1. A) DNA primase
2. B) DNA polymerase
3. C) DNA helicase
4. D) DNA ligase
5. E) Reverse transcriptase

Answer:  D

5) During library screening, PCR, Southern blotting, and other techniques, binding two pieces of DNA to each other by hydrogen bonding is called ________.

1. A) autoradiography
2. B) hybridization
3. C) DNA ligation
4. D) reverse transcription
5. E) polyadenylation

Answer:  B