Molecular Diagnostics Fundamentals Methods and Clinical Applications 1st Edition by Lela Buckingham -Test Bank

Molecular Diagnostics Fundamentals Methods and Clinical Applications 1st Edition by Lela Buckingham -Test Bank

Instant Download – Complete Test Bank With Answers

Sample Questions Are Posted Below

1. Which of the following molecules polymerize to form a support medium through which nucleic acids move?
2. A) Ethidium bromide
3. B) Ethylenediaminetetraacetic acid
4. C) Sodium dodecyl sulfate
5. D) Polyacrylamide

2. Nucleic acids migrate toward which pole in electrophoresis?
3. A) Cathode
4. B) Anode

3. A molecular technologist needs to separate oligonucleotides that are 65, 100, 250, and 350 bp in length. What concentration of acrylamide should be used for optimal separation of these fragments?
4. A) 5%
5. B) 0%
6. C) 0%
7. D) 0%

4. A very limited amount of nucleic acid, 500-1500 bp in size, needs to be analyzed in a short time (same day) with the results available immediately. Which of the following electrophoresis procedures will satisfy those conditions?
5. A) Capillary electrophoresis
6. B) Pulse–field gel electrophoresis
7. C) Field inversion gel electrophoresis
8. D) Contour-clamped homogeneous electric field

5. Which of the following is not an example of pulse–field gel electrophoresis?
6. A) Field inversion gel electrophoresis
7. B) Contour-clamped homogeneous electric field
8. C) Polyacrylamide gel electrophoresis
9. D) Transverse alternating field electrophoresis

6. A molecular biologist needs to separate and analyze a mixture of nucleic acids that are about 100,000 bp in size. Which of the following methods will yield the best results?
7. A) Capillary electrophoresis
8. B) Rotating gel electrophoresis
9. C) Polyacrylamide gel electrophoresis
10. D) Traditional agarose gel electrophoresis

7. A molecular biologist needs to separate a mixture of oligonucleotides with a resolution between bands of 1 bp. Which of the following methods should be used?
8. A) Contour-clamped homogeneous electric field
9. B) Rotating gel electrophoresis
10. C) Polyacrylamide gel electrophoresis
11. D) Traditional agarose gel electrophoresis

8. The matrix in capillary electrophoresis through which nucleic acids pass is:
9. A) silica
10. B) agarose
11. C) agarobiose
12. D) polyacrylamide

9. As compared with traditional slab gel electrophoresis, which of the following is not an advantage of capillary electrophoresis?
10. A) Analyzes smaller amounts of samples
11. B) Ability to run more than one sample together
12. C) Cost of instrumentation and labels
13. D) Real-time sample detection and analysis

10. Nucleic acids are injected into the capillary in capillary electrophoresis by which of the following methods?
11. A) Hydrostatic
12. B) Electrokinetic
13. C) Pneumatic
14. D) Vacuum

11. Which of the following molecules will migrate the fastest in capillary electrophoresis?
12. A) Large and positively charged
13. B) Small and positively charged
14. C) Large and negatively charged
15. D) Small and negatively charged

12. Which of the following molecules is added to an electrophoresis buffer for the purpose of denaturing DNA?
13. A) Ethylenediaminetetraacetic acid
14. B) Polyacrylamide
15. C) Formamide
16. D) Tris borate

13. In order to increase the density of a sample relative to the density of the buffer prior to loading the sample into a gel, which of the following is added to the sample?
14. A) Glycerol
15. B) Ethidium bromide
16. C) Formaldehyde
17. D) Tris acetate EDTA

14. A molecular technologist has run a vertical gel. While analyzing the gel, the technologist notices that the bands in the outer lanes did not run as far as similar-sized bands in the inner lanes so that the bands look like a smile on the gel. What is the explanation for this situation?
15. A) Samples were loaded into the inner wells first and got a head start moving down the gel.
16. B) A larger volume of sample was loaded into the outer wells so that it ran slower.
17. C) The electrodes in the middle of the gel were working better than at the edges of the gel.
18. D) The temperature across the gel was not constant so the inner wells were warmer.

15. Which of the following are used to make wells in a solidifying gel into which samples are loaded?
16. A) Brushes
17. B) Combs
18. C) Picks
19. D) Shovels

16. In order to monitor the progress of electrophoresis, which of the following is added to a sample that does not associate with DNA and runs ahead of the smallest fragments in the sample?
17. A) Ethidium bromide
18. B) Acrylamide
19. C) Bromophenol blue
20. D) SyBr green

17. Which of the following can be used to detect the location of DNA bands in a gel after electrophoresis?
18. A) SyBr green
19. B) Xylene cyanol green
20. C) Bromophenol blue
21. D) Acridine orange

18. Which of the following properties is true for ethidium bromide?
19. A) Emits green light when excited at 300 nm
20. B) Level of detection is 60 pg of DNA
21. C) Intercalates between nitrogen bases
22. D) Has a very low background fluorescence in agarose

19. The functions of which of the following include carrying the current and protecting the sample nucleic acids during electrophoresis?
20. A) Polyacrylamide
21. B) Formaldehyde
22. C) Ammonium persulfate
23. D) Tris acetate EDTA

Answer Key

1. D
2. B
3. C
4. A
5. C
6. B
7. C
8. A
9. C
10. B
11. D
12. C
13. A
14. D
15. B
16. C
17. A
18. C
19. D