Chapter 17 Recombinant DNA Technology

Chapter 17 Recombinant DNA Technology

Complete Chapter Questions With Answers

Sample Questions Are Posted Below

Multiple Choice Questions

1. A (n) __________ vector contains promoters that result in high-level transcription of the gene cloned within a multicloning site.
   A.shuttle
   B. chimeric
   C. expression
   D. phage

ASM Objective: 04.05 Cell genomes can be manipulated to alter cell function.
ASM Topic: Module 04 Information Flow and Genetics
Blooms Level: 2. Understand
Learning Outcome: 17.06.01 Explain the utility of expression vectors
Section: 17.06
Topic: Recombinant DNA Technology

2. Which of the following components of PCR ensures amplification of the target DNA sequence only, despite the presence of many other DNA sequences?
   A. Primers
   B.  Polymerases
   C.  dNTPs
   D.  High temperatures

ASM Objective: 04.05 Cell genomes can be manipulated to alter cell function.
ASM Topic: Module 04 Information Flow and Genetics
ASM Topic: Module 08 Microbiology Laboratory Skills
Blooms Level: 4. Analyze
Learning Outcome: 17.02.02 Explain why PCR results in the amplification of a specific DNA sequence despite many competing sequences
Section: 17.02
Topic: Basics of Genetic Engineering

3. During the investigation of a convenience store robbery, witnesses report that the perpetrator exited through a main entry door without wearing gloves. The police are holding a prime suspect in custody while samples from the door are collected and analyzed for a DNA match.  Since the door was handled by many people before the robbery, how will the forensics department distinguish the suspect’s DNA from the DNA of others using PCR?
   A. Sequence-specific primers to the suspect’s DNA will be used for PCR amplification.
   B.  Sequence-specific primers to the suspect’s DNA will be added following PCR amplification.
   C.  PCR will be used to make sequence-specific primers that are complementary to the suspect’s DNA.
   D.  Since multiple people touched the doorknob without gloves, it is impossible to use PCR to distinguish the suspect’s DNA.

ASM Objective: 04.05 Cell genomes can be manipulated to alter cell function.
ASM Topic: Module 04 Information Flow and Genetics
ASM Topic: Module 08 Microbiology Laboratory Skills
Blooms Level: 4. Analyze
Learning Outcome: 17.02.02 Explain why PCR results in the amplification of a specific DNA sequence despite many competing sequences
Section: 17.02
Topic: Basics of Genetic Engineering

True / False Questions

4. Some plasmid vectors have incorporated the regulatory sequences of the lactose operon so that the expression of the recombinant gene can be induced at the appropriate time.
   TRUE

ASM Objective: 04.05 Cell genomes can be manipulated to alter cell function.
ASM Topic: Module 04 Information Flow and Genetics
Blooms Level: 2. Understand
Learning Outcome: 17.06.01 Explain the utility of expression vectors
Section: 17.06
Topic: Recombinant DNA Technology

5. When a eukaryotic gene is expressed in a bacterium, the eukaryotic regulatory sequences should be maintained in order to achieve maximum expression of the gene.
   FALSE

ASM Objective: 04.05 Cell genomes can be manipulated to alter cell function.
ASM Topic: Module 04 Information Flow and Genetics
Blooms Level: 3. Apply
Learning Outcome: 17.06.01 Explain the utility of expression vectors
Section: 17.06
Topic: Recombinant DNA Technology